Research suffers from inefficiency, poor generalizability, and limited translational impact due to high costs, time-consuming processes, small sample sizes, and challenges in participant recruitment and reach. Research Online is a web-based platform that leverages AI, bioinstrumentation, optics, pupil dynamics, and the microvasculature to enable large-scale online physiological experiments with hundreds of participants/hour, at 1/10th the cost. The data collected contribute to building predictive models for both terrestrial and space flight applications.