Allison De Groat Daroszewski
About Allison De Groat Daroszewski
Allison De Groat Daroszewski is a Senior Associate in AAV Process Development at Abeona Therapeutics Inc., where she leads a team focused on AAV downstream processing and chromatography methods. She has a strong background in bioengineering, with experience in developing purification methods for AAV serotypes and expertise in various filtration techniques.
Work at Abeona Therapeutics
Allison De Groat Daroszewski has been employed at Abeona Therapeutics Inc. as a Senior Associate in AAV Process Development since 2018. In this role, she leads a team that specializes in AAV downstream processing, focusing on purification and scaling chromatography methods for both clinical and commercial manufacturing. Her work involves developing methods to purify various AAV serotypes on affinity resin, including novel AIM capsids.
Previous Experience at Akron Biotech
Before joining Abeona Therapeutics, Allison worked at Akron Biotech in two capacities. She served as a Manufacturing and Research Associate for 10 months in 2016 and later as a Manufacturing Process Engineer from 2016 to 2018. During her time at Akron Biotech, she contributed to the development and optimization of manufacturing processes in Boca Raton, Florida.
Education and Expertise
Allison holds a Master of Science (M.S.) in Bioengineering and Biomedical Engineering from Florida Atlantic University, which she completed from 2014 to 2015. She also earned a Graduate Certificate in Medical Anatomy and Physiology from the University of Florida College of Medicine in 2014. Additionally, she obtained a Bachelor of Science (B.S.) in Biology from the University of Florida from 2008 to 2012. Her educational background supports her advanced skills in chromatography development and purification techniques.
Technical Skills and Specializations
Allison possesses advanced skills in chromatography development, utilizing FPLC systems such as AKTA, Sepragen, and TECAN Robocolumns. She is proficient in using GE software like Unicorn. Her expertise extends to clarification techniques, including depth filtration, tangential flow filtration (both hollow fiber and flat cassette), and sterile filtration. She has also developed methods to separate empty and full AAV capsids using ion exchange resin.