Qiong Guo
About Qiong Guo
Qiong Guo is a Postdoctoral Fellow at The National Institutes of Health, specializing in cryo-electron microscopy and protein characterization. With a PhD in Biochemistry from 中国科学技术大学, Guo has expertise in optimizing protein crystals and conducting advanced molecular biology experiments.
Work at National Institutes of Health
Qiong Guo has been serving as a Postdoctoral Fellow at the National Institutes of Health since 2021. Located in Bethesda, Maryland, Guo's work focuses on advanced techniques in structural biology. This role involves significant research contributions in the field of cryo-electron microscopy (cryo-EM), where Guo prepares specimens and collects data using state-of-the-art equipment.
Education and Expertise
Qiong Guo earned a Doctor of Philosophy (PhD) in Biochemistry from 中国科学技术大学, completing the program from 2015 to 2021. This academic background provides a strong foundation in biochemistry and molecular biology, which is essential for Guo's current research. Guo has developed expertise in optimizing protein crystals and conducting complex image processing for cryo-EM.
Background
Qiong Guo's academic and professional journey is rooted in biochemistry and structural biology. With a focus on protein characterization and analysis, Guo employs various methods, including isothermal titration calorimetry and mass spectrometry, to study protein interactions and functions. This background supports ongoing research into the mechanisms of DNA translesion synthesis.
Research Techniques and Methodologies
In the realm of cryo-EM, Qiong Guo utilizes a Vitrobot for specimen preparation and a Titan Krios 300kV for data collection, equipped with a Gatan K2/K3 camera. Guo's research involves image processing using software such as RELION and CRYOSPARC, as well as model building with tools like Chimera, Phenix, and Wincoot. These methodologies are critical for determining the structures of proteins with non-canonical RNA-binding sites.
Research Focus
Qiong Guo explores the mechanisms of DNA translesion synthesis through molecular structural biology and biochemistry experiments conducted in vitro. This research aims to enhance the understanding of how proteins interact with DNA, particularly in the context of non-canonical RNA-binding sites, contributing to the broader field of molecular biology.